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Marion Bichet (Monash University, Australia) and colleagues published a protocol in Star Protocols for studying phage interactions with in vitro epithelial cell layers. They developed a simplified method for high-titer phage purification and cleanup and a comprehensive technique to look at phage-epithelial cell interaction under the microscope. They also describe the use of transwells to study microorganism transcytosis and whole-cell analysis for quantification of intracellular phage particles.