Methods for extraction and detection of Pf bacteriophage DNA from the sputum of patients with cystic fibrosis

A 2020 paper in PHAGE evaluates methods for processing sputum from patients with cystic fibrosis to detect Pf bacteriophage virion-derived nucleic acid, testing the effect of bead beating, DNase digestion and heating steps and quantifying Pseudomonas aeruginosa and Pf phage. It describes an optimized protocol for isolating DNA for PCR or sequencing-based studies.