Posts

Seeking optimised protocol for isolation of phages from seawater samples

I am working on isolation and characterisation of marine phages for anti-bio fouling activity. Our lab has assessed the influence of chlorine on different marine microorganisms as well as barnacles, and now we are looking into use of marine bacteriophage therapy for targeting the biofilms involved in microfouling which leads to macrofouling.

I am optimising a protocol for isolation of phages from marine water samples, but somewhere I am making mistakes, leading to negative results. Could anyone please share their optimised protocol for isolation of bacteriophages from sea water samples? Until now I have used a protocol which includes dilution of filtered sea water with 2X culture broth and overnight grown pure culture, but I am looking for an alternate protocol.

Please email me at atifbhu91@gmail.com if you can help.

SourceCapsid & Tail #55