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Varun Rakeshbhai Bavda (IISER, India) and colleagues published a new article on the molecular properties of mycobacteriophage D29 Holin, with an emphasis on potential for holin engineering. They demonstrated crucial amino acid regions for holin-mediated toxicity, as well as how amino acid substitution in the C-terminal region renders the holin non-toxic. They have also engineered a holin which shows higher toxicity in some strains.